Those who have positive results are called two days later for another blood test and the results of the first and the second test are evaluated together to decide whether the pregnancy is healthy or not. In a healthy pregnancy the ß-hCG values should increase by two fold in the second test. In some cases the ß-hCG values drop to zero after sometime and these are called biochemical pregnancies. An unexpected increase in the ß-hCG values on the other hand may indicate ectopic pregnancy.

Cases with expected levels of ß-hCG in the 12th and 14th day are regarded as clinical pregnancy and are asked to come back two weeks later for the first pregnancy ultrasonography. During this first ultrasonography the uterus is checked for the presence of pregnancy sacks and if present for the number of sacks present. Twins, triplets or more number of fetus can be determined during this ultrasonography.